MODULATION OF TNF-α LEVEL ON BUCCAL WOUND HEALING OF ALBINO RATS THROUGH COCOA POWDER EXTRACT

  • Maliha Shahbaz B.D.S., M.Phil, Senior Lecturer/ HOD Oral Biology, Rashid Latif Dental College, 35 Km Ferozepur Road, Lahore, Pakistan.
  • Naauman Zaheer B.D.S., M.Phil, Assistant Professor Oral Biology, CMH Lahore Medical College, Institute of Dentistry, Lahore Cantonment, Pakistan.
  • Usman Zaheer B.D.S., FCPS, Assistant Professor Orthodontics, Lahore Medical and Dental College, Lahore, Pakistan.
  • Junaid Akhtar BSC, M.Phil, Lecturer, Department of Allied Health Sciences, Sargodha Medical College, UOS, Sargodha.
Keywords: Wound healing, cocoa powder extract, polyphenols, Tumor necrosis factor alpha

Abstract

Cocoa powder has been used widely as a soothing rich chocolate drink, in baking and medical purposes for centuries. It is a rich source of polyphenols. The study was conducted on 48 Albino rats, randomly allocated into 4 groups. The control group was considered as group I in which rats were given normal saline along with standard diet while rats in the experimental group II, III and IV were given low, medium and high dosage of cocoa extract respectively dissolved in water by oral gavage daily along with standard rat chow diet. A 3mm surgical excisional wound was created on both left and right buccal mucosa of rats of all four groups with the help of punch-biopsy procedure. Tissue samples were obtained by sacrificing the rats on 3rd, 7th and 14th day after the surgical procedure. Gene expression of Tumor necrosis factor alpha (TNF-α) was evaluated through Real Time PCR (qPCR) and levels were measured quantitatively. The study concluded that cocoa powder extract in high dosage did have a positive effect in modulating the pro-inflammatory cytokines by decreasing gene expression of TNF-α and reducing the inflammatory process.

Published
2018-09-10
How to Cite
Shahbaz, M., Zaheer, N., Zaheer, U., & Akhtar, J. (2018). MODULATION OF TNF-α LEVEL ON BUCCAL WOUND HEALING OF ALBINO RATS THROUGH COCOA POWDER EXTRACT. Pakistan Oral & Dental Journal, 38(2), 182-186. Retrieved from http://podj.com.pk/index.php/podj/article/view/185
Section
Oral Biology